Detailed information of TA system TA051287

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4041801..4042160
• Replicon: chromosome
• Accession: NZ_CP010191
• Organism: Escherichia coli strain M8

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG57_RS20810
• Protein ID: WP_001317028.1
• Coordinates: 4041801..4041995 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4041994..4042160 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG57_RS20785	4037499..4037720	+	222	WP_000560223.1	killing protein KilR	-
RG57_RS20790	4037720..4037890	+	171	WP_001352098.1	conserved protein, Rac prophage	-
RG57_RS20795	4037965..4038240	+	276	WP_000632297.1	protein RacC	-
RG57_RS20800	4038342..4040942	+	2601	WP_073535232.1	exodeoxyribonuclease VIII	-
RG57_RS20805	4040935..4041744	+	810	WP_000166319.1	recombination protein RecT	-
RG57_RS20810	4041801..4041995	+	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG57_RS20815	4041988..4042185	+	198	WP_024170988.1	DUF1187 family protein	-
-	4041994..4042160	-	167	NuclAT_1	-	Antitoxin
-	4041994..4042160	-	167	NuclAT_1	-	Antitoxin
-	4041994..4042160	-	167	NuclAT_1	-	Antitoxin
-	4041994..4042160	-	167	NuclAT_1	-	Antitoxin
RG57_RS20820	4042264..4042479	+	216	WP_000079604.1	excisionase XisR	-
RG57_RS20825	4042481..4043716	+	1236	WP_000040852.1	site-specific integrase	-
RG57_RS20830	4043768..4044703	+	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG57_RS20835	4044832..4046205	-	1374	WP_000123738.1	ATP-dependent RNA helicase DbpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3995066..4043716	48650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T51287 WP_001317028.1 NZ_CP010191:4041801-4041995 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T51287 NZ_CP010191:4041801-4041995 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 167 bp

>AT51287 NZ_CP010191:c4042160-4041994 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin