Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3361892..3362394 Replicon chromosome
Accession NZ_CP010191
Organism Escherichia coli strain M8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG57_RS17225 Protein ID WP_000767829.1
Coordinates 3362140..3362394 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG57_RS17220 Protein ID WP_001259255.1
Coordinates 3361892..3362143 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG57_RS17190 3357069..3358136 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG57_RS17195 3358136..3359206 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG57_RS17200 3359203..3360507 - 1305 WP_001317939.1 histidinol dehydrogenase -
RG57_RS17205 3360513..3361412 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG57_RS17215 3361558..3361608 - 51 WP_001364200.1 his operon leader peptide -
RG57_RS17220 3361892..3362143 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG57_RS17225 3362140..3362394 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG57_RS17230 3362477..3363301 + 825 WP_000754758.1 SDR family oxidoreductase -
RG57_RS17235 3363347..3364276 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG57_RS27285 3364491..3364553 + 63 WP_010723108.1 membrane protein YoeI -
RG57_RS17240 3364543..3365901 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG57_RS17245 3366080..3367138 + 1059 WP_000492346.1 transport protein YeeE -
RG57_RS17250 3367152..3367379 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51276 WP_000767829.1 NZ_CP010191:3362140-3362394 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51276 NZ_CP010191:3362140-3362394 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51276 WP_001259255.1 NZ_CP010191:3361892-3362143 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51276 NZ_CP010191:3361892-3362143 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References