Detailed information of TA system TA051236

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4754507..4754866
• Replicon: chromosome
• Accession: NZ_CP010183
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG55_RS24935
• Protein ID: WP_001317028.1
• Coordinates: 4754507..4754701 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4754700..4754866 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS24910	4750205..4750426	+	222	WP_000560224.1	killing protein KilR	-
RG55_RS24915	4750426..4750596	+	171	WP_001352098.1	conserved protein, Rac prophage	-
RG55_RS24920	4750671..4750946	+	276	WP_024251429.1	hypothetical protein	-
RG55_RS24925	4751048..4753648	+	2601	WP_000105136.1	exodeoxyribonuclease VIII	-
RG55_RS24930	4753641..4754450	+	810	WP_000166319.1	recombination protein RecT	-
RG55_RS24935	4754507..4754701	+	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG55_RS24940	4754694..4754891	+	198	WP_024170988.1	DUF1187 family protein	-
-	4754700..4754866	-	167	NuclAT_1	-	Antitoxin
-	4754700..4754866	-	167	NuclAT_1	-	Antitoxin
-	4754700..4754866	-	167	NuclAT_1	-	Antitoxin
-	4754700..4754866	-	167	NuclAT_1	-	Antitoxin
RG55_RS24945	4754970..4755185	+	216	WP_000079604.1	excisionase XisR	-
RG55_RS24950	4755187..4756422	+	1236	WP_000040852.1	site-specific integrase	-
RG55_RS24955	4756474..4757409	+	936	WP_053264959.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG55_RS24960	4757538..4758911	-	1374	WP_000123739.1	ATP-dependent RNA helicase DbpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4707325..4760372	53047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T51236 WP_001317028.1 NZ_CP010183:4754507-4754701 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T51236 NZ_CP010183:4754507-4754701 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 167 bp

>AT51236 NZ_CP010183:c4754866-4754700 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin