Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4013437..4013939 Replicon chromosome
Accession NZ_CP010183
Organism Escherichia coli strain M3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG55_RS20845 Protein ID WP_000767829.1
Coordinates 4013685..4013939 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG55_RS20840 Protein ID WP_001259255.1
Coordinates 4013437..4013688 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG55_RS20810 4008615..4009682 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG55_RS20815 4009682..4010752 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
RG55_RS20820 4010749..4012053 - 1305 WP_001593467.1 histidinol dehydrogenase -
RG55_RS20825 4012059..4012958 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG55_RS20835 4013104..4013154 - 51 WP_001364200.1 his operon leader peptide -
RG55_RS20840 4013437..4013688 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG55_RS20845 4013685..4013939 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG55_RS20850 4014022..4014846 + 825 WP_000754758.1 SDR family oxidoreductase -
RG55_RS20855 4014892..4015821 + 930 WP_000803370.1 LysR family transcriptional regulator -
RG55_RS28040 4016036..4016098 + 63 WP_010723108.1 membrane protein YoeI -
RG55_RS20860 4016088..4017446 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG55_RS20865 4017625..4018683 + 1059 WP_000492346.1 transport protein YeeE -
RG55_RS20870 4018697..4018924 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51224 WP_000767829.1 NZ_CP010183:4013685-4013939 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51224 NZ_CP010183:4013685-4013939 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51224 WP_001259255.1 NZ_CP010183:4013437-4013688 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51224 NZ_CP010183:4013437-4013688 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References