51224

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4013437..4013939 Replicon chromosome
Accession NZ_CP010183
Organism Escherichia coli strain M3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG55_RS20845 Protein ID WP_000767829.1
Coordinates 4013685..4013939 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG55_RS20840 Protein ID WP_001259255.1
Coordinates 4013437..4013688 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG55_RS20810 4008615..4009682 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG55_RS20815 4009682..4010752 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
RG55_RS20820 4010749..4012053 - 1305 WP_001593467.1 histidinol dehydrogenase -
RG55_RS20825 4012059..4012958 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG55_RS20835 4013104..4013154 - 51 WP_001364200.1 his operon leader peptide -
RG55_RS20840 4013437..4013688 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG55_RS20845 4013685..4013939 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG55_RS20850 4014022..4014846 + 825 WP_000754758.1 SDR family oxidoreductase -
RG55_RS20855 4014892..4015821 + 930 WP_000803370.1 LysR family transcriptional regulator -
RG55_RS28040 4016036..4016098 + 63 WP_010723108.1 membrane protein YoeI -
RG55_RS20860 4016088..4017446 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG55_RS20865 4017625..4018683 + 1059 WP_000492346.1 transport protein YeeE -
RG55_RS20870 4018697..4018924 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51224 WP_000767829.1 NZ_CP010183:4013685-4013939 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51224 NZ_CP010183:4013685-4013939 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51224 WP_001259255.1 NZ_CP010183:4013437-4013688 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51224 NZ_CP010183:4013437-4013688 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References