Detailed information of TA system TA051219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2741647..2742446
• Replicon: chromosome
• Accession: NZ_CP010183
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: RG55_RS14350
• Protein ID: WP_000347253.1
• Coordinates: 2741647..2742111 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: RG55_RS14355
• Protein ID: WP_024251361.1
• Coordinates: 2742111..2742446 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS14315	2736648..2737082	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
RG55_RS14320	2737100..2737978	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
RG55_RS14325	2737968..2738747	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
RG55_RS14330	2738758..2739231	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
RG55_RS14335	2739254..2740534	-	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
RG55_RS14345	2740783..2741592	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
RG55_RS14350	2741647..2742111	-	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
RG55_RS14355	2742111..2742446	-	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
RG55_RS14360	2742595..2744166	-	1572	WP_001273766.1	galactarate dehydratase	-
RG55_RS14365	2744541..2745875	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
RG55_RS14370	2745891..2746661	+	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T51219 WP_000347253.1 NZ_CP010183:c2742111-2741647 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T51219 NZ_CP010183:c2742111-2741647 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT51219 WP_024251361.1 NZ_CP010183:c2742446-2742111 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT51219 NZ_CP010183:c2742446-2742111 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA