51218

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1955548..1956150 Replicon chromosome
Accession NZ_CP010183
Organism Escherichia coli strain M3

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag RG55_RS10265 Protein ID WP_000897305.1
Coordinates 1955839..1956150 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag RG55_RS10260 Protein ID WP_000356397.1
Coordinates 1955548..1955838 (-) Length 97 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG55_RS10225 1951474..1952376 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
RG55_RS10230 1952373..1953008 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
RG55_RS10235 1953005..1953934 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
RG55_RS10245 1954264..1954506 - 243 WP_001086388.1 hypothetical protein -
RG55_RS10250 1954725..1954943 - 219 WP_001297075.1 ribbon-helix-helix domain-containing protein -
RG55_RS10260 1955548..1955838 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
RG55_RS10265 1955839..1956150 - 312 WP_000897305.1 hypothetical protein Toxin
RG55_RS10270 1956379..1957287 + 909 WP_001162704.1 alpha/beta hydrolase -
RG55_RS10275 1957351..1958292 - 942 WP_001395735.1 fatty acid biosynthesis protein FabY -
RG55_RS10280 1958337..1958774 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
RG55_RS10285 1958771..1959643 - 873 WP_000920762.1 virulence factor BrkB family protein -
RG55_RS10290 1959637..1960236 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(36-85)


Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T51218 WP_000897305.1 NZ_CP010183:c1956150-1955839 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T51218 NZ_CP010183:c1956150-1955839 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT51218 WP_000356397.1 NZ_CP010183:c1955838-1955548 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT51218 NZ_CP010183:c1955838-1955548 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7


Antitoxin

Source ID Structure

References