Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | higBA (relBE)/HTH(antitoxin) |
Location | 1955548..1956150 | Replicon | chromosome |
Accession | NZ_CP010183 | ||
Organism | Escherichia coli strain M3 |
Toxin (Protein)
Gene name | higB | Uniprot ID | U9XIS6 |
Locus tag | RG55_RS10265 | Protein ID | WP_000897305.1 |
Coordinates | 1955839..1956150 (-) | Length | 104 a.a. |
Antitoxin (Protein)
Gene name | higA | Uniprot ID | - |
Locus tag | RG55_RS10260 | Protein ID | WP_000356397.1 |
Coordinates | 1955548..1955838 (-) | Length | 97 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
RG55_RS10225 | 1951474..1952376 | + | 903 | WP_000331377.1 | formate dehydrogenase O subunit beta | - |
RG55_RS10230 | 1952373..1953008 | + | 636 | WP_000829013.1 | formate dehydrogenase cytochrome b556 subunit | - |
RG55_RS10235 | 1953005..1953934 | + | 930 | WP_000027708.1 | formate dehydrogenase accessory protein FdhE | - |
RG55_RS10245 | 1954264..1954506 | - | 243 | WP_001086388.1 | hypothetical protein | - |
RG55_RS10250 | 1954725..1954943 | - | 219 | WP_001297075.1 | ribbon-helix-helix domain-containing protein | - |
RG55_RS10260 | 1955548..1955838 | - | 291 | WP_000356397.1 | helix-turn-helix domain-containing protein | Antitoxin |
RG55_RS10265 | 1955839..1956150 | - | 312 | WP_000897305.1 | hypothetical protein | Toxin |
RG55_RS10270 | 1956379..1957287 | + | 909 | WP_001162704.1 | alpha/beta hydrolase | - |
RG55_RS10275 | 1957351..1958292 | - | 942 | WP_001395735.1 | fatty acid biosynthesis protein FabY | - |
RG55_RS10280 | 1958337..1958774 | - | 438 | WP_000560983.1 | D-tyrosyl-tRNA(Tyr) deacylase | - |
RG55_RS10285 | 1958771..1959643 | - | 873 | WP_000920762.1 | virulence factor BrkB family protein | - |
RG55_RS10290 | 1959637..1960236 | - | 600 | WP_001295269.1 | glucose-1-phosphatase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12190.19 Da Isoelectric Point: 9.7791
>T51218 WP_000897305.1 NZ_CP010183:c1956150-1955839 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
Download Length: 312 bp
>T51218 NZ_CP010183:c1956150-1955839 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 97 a.a. Molecular weight: 10846.43 Da Isoelectric Point: 10.8907
>AT51218 WP_000356397.1 NZ_CP010183:c1955838-1955548 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
Download Length: 291 bp
>AT51218 NZ_CP010183:c1955838-1955548 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|