Detailed information of TA system TA051204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2976712..2977314
• Replicon: chromosome
• Accession: NZ_CP010180
• Organism: Escherichia coli strain M1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG54_RS15640
• Protein ID: WP_000897305.1
• Coordinates: 2976712..2977023 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG54_RS15645
• Protein ID: WP_000356397.1
• Coordinates: 2977024..2977314 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG54_RS15615	2972626..2973225	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG54_RS15620	2973219..2974091	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG54_RS15625	2974088..2974525	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG54_RS15630	2974570..2975511	+	942	WP_001395735.1	fatty acid biosynthesis protein FabY	-
RG54_RS15635	2975575..2976483	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG54_RS15640	2976712..2977023	+	312	WP_000897305.1	hypothetical protein	Toxin
RG54_RS15645	2977024..2977314	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG54_RS15655	2977919..2978137	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
RG54_RS15660	2978356..2978598	+	243	WP_001086388.1	hypothetical protein	-
RG54_RS15670	2978928..2979857	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG54_RS15675	2979854..2980489	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG54_RS15680	2980486..2981388	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51204 WP_000897305.1 NZ_CP010180:2976712-2977023 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51204 NZ_CP010180:2976712-2977023 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51204 WP_000356397.1 NZ_CP010180:2977024-2977314 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51204 NZ_CP010180:2977024-2977314 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7