Detailed information of TA system TA051203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2189460..2190259
• Replicon: chromosome
• Accession: NZ_CP010180
• Organism: Escherichia coli strain M1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: RG54_RS11540
• Protein ID: WP_000347253.1
• Coordinates: 2189795..2190259 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: RG54_RS11535
• Protein ID: WP_024251361.1
• Coordinates: 2189460..2189795 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG54_RS11520	2185245..2186015	-	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-
RG54_RS11525	2186031..2187365	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
RG54_RS11530	2187740..2189311	+	1572	WP_001273766.1	galactarate dehydratase	-
RG54_RS11535	2189460..2189795	+	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
RG54_RS11540	2189795..2190259	+	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
RG54_RS11545	2190314..2191123	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
RG54_RS11555	2191372..2192652	+	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
RG54_RS11560	2192675..2193148	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
RG54_RS11565	2193159..2193938	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
RG54_RS11570	2193928..2194806	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
RG54_RS11575	2194824..2195258	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2178585..2190259	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T51203 WP_000347253.1 NZ_CP010180:2189795-2190259 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T51203 NZ_CP010180:2189795-2190259 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT51203 WP_024251361.1 NZ_CP010180:2189460-2189795 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT51203 NZ_CP010180:2189460-2189795 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA