Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 918198..918700 Replicon chromosome
Accession NZ_CP010180
Organism Escherichia coli strain M1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG54_RS05040 Protein ID WP_000767829.1
Coordinates 918198..918452 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG54_RS05045 Protein ID WP_001259255.1
Coordinates 918449..918700 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG54_RS05015 913213..913440 - 228 WP_000985270.1 sulfurtransferase TusA family protein -
RG54_RS05020 913454..914512 - 1059 WP_000492346.1 transport protein YeeE -
RG54_RS05025 914691..916049 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG54_RS27800 916039..916101 - 63 WP_010723108.1 membrane protein YoeI -
RG54_RS05030 916316..917245 - 930 WP_000803370.1 LysR family transcriptional regulator -
RG54_RS05035 917291..918115 - 825 WP_000754758.1 SDR family oxidoreductase -
RG54_RS05040 918198..918452 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG54_RS05045 918449..918700 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG54_RS05050 918983..919033 + 51 WP_001364200.1 his operon leader peptide -
RG54_RS05060 919179..920078 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG54_RS05065 920084..921388 + 1305 WP_001593467.1 histidinol dehydrogenase -
RG54_RS05070 921385..922455 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
RG54_RS05075 922455..923522 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51198 WP_000767829.1 NZ_CP010180:c918452-918198 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51198 NZ_CP010180:c918452-918198 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51198 WP_001259255.1 NZ_CP010180:c918700-918449 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51198 NZ_CP010180:c918700-918449 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References