Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) ralRA/-
Location 177271..177630 Replicon chromosome
Accession NZ_CP010180
Organism Escherichia coli strain M1

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag RG54_RS00950 Protein ID WP_001317028.1
Coordinates 177436..177630 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 177271..177437 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG54_RS00925 173226..174599 + 1374 WP_000123739.1 ATP-dependent RNA helicase DbpA -
RG54_RS00930 174728..175663 - 936 WP_053264959.1 tRNA 2-thiocytidine(32) synthetase TtcA -
RG54_RS00935 175715..176950 - 1236 WP_000040852.1 site-specific integrase -
RG54_RS00940 176952..177167 - 216 WP_000079604.1 excisionase XisR -
RG54_RS00945 177246..177443 - 198 WP_024170988.1 DUF1187 family protein -
- 177271..177437 + 167 NuclAT_1 - Antitoxin
- 177271..177437 + 167 NuclAT_1 - Antitoxin
- 177271..177437 + 167 NuclAT_1 - Antitoxin
- 177271..177437 + 167 NuclAT_1 - Antitoxin
RG54_RS00950 177436..177630 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
RG54_RS00955 177687..178496 - 810 WP_000166319.1 recombination protein RecT -
RG54_RS00960 178489..181089 - 2601 WP_000105136.1 exodeoxyribonuclease VIII -
RG54_RS00965 181191..181466 - 276 WP_024251429.1 hypothetical protein -
RG54_RS00970 181541..181711 - 171 WP_001352098.1 conserved protein, Rac prophage -
RG54_RS00975 181711..181932 - 222 WP_000560224.1 killing protein KilR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 175715..224812 49097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-54)


Sequences


Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T51183 WP_001317028.1 NZ_CP010180:c177630-177436 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T51183 NZ_CP010180:c177630-177436 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 167 bp

>AT51183 NZ_CP010180:177271-177437 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7


Antitoxin

Download structure file

References