51179

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 44875..45602 Replicon plasmid A
Accession NZ_CP010179
Organism Escherichia coli strain H15

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag RG53_RS24665 Protein ID WP_000558568.1
Coordinates 44875..45186 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag RG53_RS24670 Protein ID WP_000990392.1
Coordinates 45183..45602 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG53_RS24645 41389..42108 + 720 WP_001102107.1 (Fe-S)-binding protein -
RG53_RS24650 42119..43546 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
RG53_RS24655 43539..44234 + 696 WP_000370412.1 lactate utilization protein C -
RG53_RS24660 44284..44664 - 381 Protein_50 gluconate permease -
RG53_RS24665 44875..45186 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
RG53_RS24670 45183..45602 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
RG53_RS24675 45639..46841 - 1203 WP_000753105.1 hypothetical protein -
RG53_RS24680 46834..47163 - 330 WP_000121260.1 hypothetical protein -
RG53_RS24685 47160..47819 - 660 WP_000078540.1 hypothetical protein -
RG53_RS24695 48243..48836 - 594 WP_042111017.1 hypothetical protein -
RG53_RS24700 49152..49505 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
RG53_RS24705 49553..49915 + 363 WP_000783215.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..116417 116417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T51179 WP_000558568.1 NZ_CP010179:44875-45186 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T51179 NZ_CP010179:44875-45186 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGATAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT51179 WP_000990392.1 NZ_CP010179:45183-45602 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT51179 NZ_CP010179:45183-45602 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References