Detailed information of TA system TA051177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3922939..3923541
• Replicon: chromosome
• Accession: NZ_CP010178
• Organism: Escherichia coli strain H15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG53_RS20310
• Protein ID: WP_000897305.1
• Coordinates: 3922939..3923250 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG53_RS20315
• Protein ID: WP_000356397.1
• Coordinates: 3923251..3923541 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG53_RS20280	3917969..3918754	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG53_RS20285	3918853..3919452	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG53_RS20290	3919446..3920318	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG53_RS20295	3920315..3920752	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG53_RS20300	3920797..3921738	+	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
RG53_RS20305	3921802..3922710	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG53_RS20310	3922939..3923250	+	312	WP_000897305.1	hypothetical protein	Toxin
RG53_RS20315	3923251..3923541	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG53_RS20325	3924146..3924364	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
RG53_RS20330	3924583..3924825	+	243	WP_001086388.1	hypothetical protein	-
RG53_RS20340	3925155..3926084	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG53_RS20345	3926081..3926716	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG53_RS20350	3926713..3927615	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51177 WP_000897305.1 NZ_CP010178:3922939-3923250 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51177 NZ_CP010178:3922939-3923250 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51177 WP_000356397.1 NZ_CP010178:3923251-3923541 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51177 NZ_CP010178:3923251-3923541 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7