Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1898618..1899120 Replicon chromosome
Accession NZ_CP010178
Organism Escherichia coli strain H15

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG53_RS09950 Protein ID WP_000767829.1
Coordinates 1898618..1898872 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG53_RS09955 Protein ID WP_001259255.1
Coordinates 1898869..1899120 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG53_RS09925 1893633..1893860 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG53_RS09930 1893874..1894932 - 1059 WP_000492346.1 transport protein YeeE -
RG53_RS09935 1895111..1896469 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG53_RS25805 1896459..1896521 - 63 WP_010723108.1 membrane protein YoeI -
RG53_RS09940 1896736..1897665 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG53_RS09945 1897711..1898535 - 825 WP_000754749.1 SDR family oxidoreductase -
RG53_RS09950 1898618..1898872 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG53_RS09955 1898869..1899120 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG53_RS09960 1899403..1899453 + 51 WP_001364200.1 his operon leader peptide -
RG53_RS09970 1899599..1900498 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG53_RS09975 1900504..1901808 + 1305 WP_001349967.1 histidinol dehydrogenase -
RG53_RS09980 1901805..1902875 + 1071 WP_000108955.1 histidinol-phosphate transaminase -
RG53_RS09985 1902875..1903942 + 1068 WP_000080108.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51171 WP_000767829.1 NZ_CP010178:c1898872-1898618 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51171 NZ_CP010178:c1898872-1898618 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51171 WP_001259255.1 NZ_CP010178:c1899120-1898869 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51171 NZ_CP010178:c1899120-1898869 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References