Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2023864..2024366 Replicon chromosome
Accession NZ_CP010176
Organism Escherichia coli strain H10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG51_RS10650 Protein ID WP_000767829.1
Coordinates 2023864..2024118 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG51_RS10655 Protein ID WP_001259255.1
Coordinates 2024115..2024366 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG51_RS10625 2018879..2019106 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG51_RS10630 2019120..2020178 - 1059 WP_000492340.1 transport protein YeeE -
RG51_RS10635 2020357..2021715 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG51_RS25500 2021705..2021767 - 63 WP_010723108.1 membrane protein YoeI -
RG51_RS10640 2021982..2022911 - 930 WP_000803360.1 LysR family transcriptional regulator -
RG51_RS10645 2022957..2023781 - 825 WP_000754749.1 SDR family oxidoreductase -
RG51_RS10650 2023864..2024118 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG51_RS10655 2024115..2024366 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG51_RS10660 2024649..2024699 + 51 WP_001364200.1 his operon leader peptide -
RG51_RS10670 2024845..2025744 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG51_RS10675 2025750..2027054 + 1305 WP_045172205.1 histidinol dehydrogenase -
RG51_RS10680 2027051..2028121 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
RG51_RS10685 2028121..2029188 + 1068 WP_000080063.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51123 WP_000767829.1 NZ_CP010176:c2024118-2023864 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51123 NZ_CP010176:c2024118-2023864 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51123 WP_001259255.1 NZ_CP010176:c2024366-2024115 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51123 NZ_CP010176:c2024366-2024115 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References