Detailed information of TA system TA051103

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60147..60417
• Replicon: plasmid B
• Accession: NZ_CP010174
• Organism: Escherichia coli strain H8

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG50_RS26930
• Protein ID: WP_001312861.1
• Coordinates: 60259..60417 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60147..60210 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG50_RS26900	55858..56385	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
RG50_RS26905	56443..56676	+	234	WP_000006003.1	DUF905 family protein	-
RG50_RS26910	56737..58760	+	2024	Protein_70	ParB/RepB/Spo0J family partition protein	-
RG50_RS26915	58829..59263	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
RG50_RS26920	59260..59979	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	59991..60215	+	225	NuclAT_0	-	-
-	59991..60215	+	225	NuclAT_0	-	-
-	59991..60215	+	225	NuclAT_0	-	-
-	59991..60215	+	225	NuclAT_0	-	-
RG50_RS28930	60000..60179	-	180	WP_001309233.1	hypothetical protein	-
-	60147..60210	-	64	-	-	Antitoxin
RG50_RS26930	60259..60417	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG50_RS29125	60655..61032	-	378	Protein_75	hypothetical protein	-
RG50_RS26950	61332..61628	+	297	WP_001272251.1	hypothetical protein	-
RG50_RS26955	61739..62560	+	822	WP_001234445.1	DUF945 domain-containing protein	-
RG50_RS26960	62857..63459	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
RG50_RS26965	63782..64165	+	384	WP_001354030.1	relaxosome protein TraM	-
RG50_RS26970	64359..65030	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
RG50_RS26975	65167..65394	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(3)-IIa / erm(B) / blaTEM-1B / blaCTX-M-15	-	1..106274	106274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51103 WP_001312861.1 NZ_CP010174:60259-60417 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51103 NZ_CP010174:60259-60417 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT51103 NZ_CP010174:c60210-60147 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin