Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3058128..3058630 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG50_RS15815 Protein ID WP_000767829.1
Coordinates 3058376..3058630 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG50_RS15810 Protein ID WP_001259255.1
Coordinates 3058128..3058379 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS15780 3053306..3054373 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG50_RS15785 3054373..3055443 - 1071 WP_001518029.1 histidinol-phosphate transaminase -
RG50_RS15790 3055440..3056744 - 1305 WP_001367667.1 histidinol dehydrogenase -
RG50_RS15795 3056750..3057649 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
RG50_RS15805 3057795..3057845 - 51 WP_001364200.1 his operon leader peptide -
RG50_RS15810 3058128..3058379 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG50_RS15815 3058376..3058630 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG50_RS15820 3058713..3059537 + 825 WP_000754737.1 SDR family oxidoreductase -
RG50_RS15825 3059583..3060512 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG50_RS28330 3060727..3060789 + 63 WP_010723108.1 membrane protein YoeI -
RG50_RS15830 3060779..3062137 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG50_RS15835 3062316..3063374 + 1059 WP_000492333.1 transport protein YeeE -
RG50_RS15840 3063388..3063615 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51088 WP_000767829.1 NZ_CP010172:3058376-3058630 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51088 NZ_CP010172:3058376-3058630 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51088 WP_001259255.1 NZ_CP010172:3058128-3058379 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51088 NZ_CP010172:3058128-3058379 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References