51088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3058128..3058630 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG50_RS15815 Protein ID WP_000767829.1
Coordinates 3058376..3058630 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG50_RS15810 Protein ID WP_001259255.1
Coordinates 3058128..3058379 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS15780 3053306..3054373 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG50_RS15785 3054373..3055443 - 1071 WP_001518029.1 histidinol-phosphate transaminase -
RG50_RS15790 3055440..3056744 - 1305 WP_001367667.1 histidinol dehydrogenase -
RG50_RS15795 3056750..3057649 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
RG50_RS15805 3057795..3057845 - 51 WP_001364200.1 his operon leader peptide -
RG50_RS15810 3058128..3058379 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG50_RS15815 3058376..3058630 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG50_RS15820 3058713..3059537 + 825 WP_000754737.1 SDR family oxidoreductase -
RG50_RS15825 3059583..3060512 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG50_RS28330 3060727..3060789 + 63 WP_010723108.1 membrane protein YoeI -
RG50_RS15830 3060779..3062137 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG50_RS15835 3062316..3063374 + 1059 WP_000492333.1 transport protein YeeE -
RG50_RS15840 3063388..3063615 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51088 WP_000767829.1 NZ_CP010172:3058376-3058630 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51088 NZ_CP010172:3058376-3058630 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51088 WP_001259255.1 NZ_CP010172:3058128-3058379 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51088 NZ_CP010172:3058128-3058379 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References