Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2254378..2255105 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag RG50_RS11865 Protein ID WP_001521139.1
Coordinates 2254378..2254689 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag RG50_RS11870 Protein ID WP_000126294.1
Coordinates 2254686..2255105 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS11840 2250313..2252022 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
RG50_RS11845 2252032..2252574 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
RG50_RS11850 2252574..2253341 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
RG50_RS11855 2253338..2253748 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
RG50_RS11860 2253741..2254211 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
RG50_RS11865 2254378..2254689 + 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
RG50_RS11870 2254686..2255105 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
RG50_RS11875 2255184..2256608 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
RG50_RS11880 2256617..2258074 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
RG50_RS11885 2258334..2259344 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
RG50_RS11890 2259493..2260020 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T51087 WP_001521139.1 NZ_CP010172:2254378-2254689 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T51087 NZ_CP010172:2254378-2254689 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT51087 WP_000126294.1 NZ_CP010172:2254686-2255105 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT51087 NZ_CP010172:2254686-2255105 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829L2T9


Antitoxin

Source ID Structure

References