Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 108580..109274 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag RG50_RS00665 Protein ID WP_001263489.1
Coordinates 108580..108978 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag RG50_RS00670 Protein ID WP_000554758.1
Coordinates 108981..109274 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 104168..104248 - 81 NuclAT_11 - -
- 104168..104248 - 81 NuclAT_11 - -
- 104168..104248 - 81 NuclAT_11 - -
- 104168..104248 - 81 NuclAT_11 - -
RG50_RS00635 104844..105302 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
RG50_RS00640 105563..107020 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
RG50_RS00645 107077..107598 - 522 Protein_105 peptide chain release factor H -
RG50_RS00650 107594..107800 - 207 Protein_106 RtcB family protein -
RG50_RS00660 108118..108570 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
RG50_RS00665 108580..108978 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
RG50_RS00670 108981..109274 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
RG50_RS00675 109326..110381 - 1056 WP_001226164.1 DNA polymerase IV -
RG50_RS00680 110452..111237 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
RG50_RS00685 111209..112921 + 1713 Protein_112 flagellar biosynthesis protein FlhA -
RG50_RS00695 113145..113642 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T51077 WP_001263489.1 NZ_CP010172:c108978-108580 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T51077 NZ_CP010172:c108978-108580 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT51077 WP_000554758.1 NZ_CP010172:c109274-108981 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT51077 NZ_CP010172:c109274-108981 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References