Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4737712..4738214 Replicon chromosome
Accession NZ_CP010171
Organism Escherichia coli strain H7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG49_RS25125 Protein ID WP_000767829.1
Coordinates 4737960..4738214 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG49_RS25120 Protein ID WP_001259255.1
Coordinates 4737712..4737963 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG49_RS25090 4732890..4733957 - 1068 WP_032208185.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG49_RS25095 4733957..4735027 - 1071 WP_032208187.1 histidinol-phosphate transaminase -
RG49_RS25100 4735024..4736328 - 1305 WP_021569431.1 histidinol dehydrogenase -
RG49_RS25105 4736334..4737233 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
RG49_RS25115 4737379..4737429 - 51 WP_001364200.1 his operon leader peptide -
RG49_RS25120 4737712..4737963 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG49_RS25125 4737960..4738214 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG49_RS25130 4738297..4739121 + 825 WP_021569430.1 SDR family oxidoreductase -
RG49_RS25135 4739167..4740096 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG49_RS26295 4740311..4740373 + 63 WP_010723108.1 membrane protein YoeI -
RG49_RS25140 4740363..4741721 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG49_RS25145 4741900..4742958 + 1059 WP_000492312.1 transport protein YeeE -
RG49_RS25150 4742972..4743199 + 228 WP_021569429.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51076 WP_000767829.1 NZ_CP010171:4737960-4738214 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51076 NZ_CP010171:4737960-4738214 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51076 WP_001259255.1 NZ_CP010171:4737712-4737963 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51076 NZ_CP010171:4737712-4737963 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References