Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3528048..3528847 Replicon chromosome
Accession NZ_CP010171
Organism Escherichia coli strain H7

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag RG49_RS19020 Protein ID WP_000347273.1
Coordinates 3528048..3528512 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag RG49_RS19025 Protein ID WP_001307405.1
Coordinates 3528512..3528847 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG49_RS18985 3523049..3523483 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
RG49_RS18990 3523501..3524379 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
RG49_RS18995 3524369..3525148 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
RG49_RS19000 3525159..3525632 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
RG49_RS19005 3525655..3526935 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
RG49_RS19015 3527184..3527993 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
RG49_RS19020 3528048..3528512 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
RG49_RS19025 3528512..3528847 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
RG49_RS19030 3528996..3530567 - 1572 WP_001273753.1 galactarate dehydratase -
RG49_RS19035 3530942..3532276 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
RG49_RS19040 3532292..3533062 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T51071 WP_000347273.1 NZ_CP010171:c3528512-3528048 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T51071 NZ_CP010171:c3528512-3528048 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT51071 WP_001307405.1 NZ_CP010171:c3528847-3528512 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT51071 NZ_CP010171:c3528847-3528512 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References