Detailed information of TA system TA051068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1882710..1883404
• Replicon: chromosome
• Accession: NZ_CP010171
• Organism: Escherichia coli strain H7

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG49_RS10510
• Protein ID: WP_001263493.1
• Coordinates: 1882710..1883108 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG49_RS10515
• Protein ID: WP_000554757.1
• Coordinates: 1883111..1883404 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG49_RS10480	1877710..1878954	-	1245	WP_000189541.1	esterase FrsA	-
-	1878370..1878450	-	81	NuclAT_11	-	-
-	1878370..1878450	-	81	NuclAT_11	-	-
-	1878370..1878450	-	81	NuclAT_11	-	-
-	1878370..1878450	-	81	NuclAT_11	-	-
RG49_RS10485	1879046..1879504	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG49_RS10490	1879765..1881222	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG49_RS10495	1881279..1881800	-	522	Protein_1859	peptide chain release factor H	-
RG49_RS10500	1881799..1882002	-	204	Protein_1860	RNA ligase RtcB family protein	-
RG49_RS10505	1882248..1882700	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG49_RS10510	1882710..1883108	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG49_RS10515	1883111..1883404	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG49_RS10520	1883456..1884511	-	1056	WP_001226164.1	DNA polymerase IV	-
RG49_RS10525	1884582..1885367	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
RG49_RS10530	1885339..1887051	+	1713	Protein_1866	flagellar biosynthesis protein FlhA	-
RG49_RS10540	1887275..1887772	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T51068 WP_001263493.1 NZ_CP010171:c1883108-1882710 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51068 NZ_CP010171:c1883108-1882710 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51068 WP_000554757.1 NZ_CP010171:c1883404-1883111 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51068 NZ_CP010171:c1883404-1883111 [Escherichia coli]
ATGCACCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6