Detailed information of TA system TA051054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3987279..3987973
• Replicon: chromosome
• Accession: NZ_CP010170
• Organism: Escherichia coli strain H6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: RG48_RS20880
• Protein ID: WP_073464912.1
• Coordinates: 3987279..3987677 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG48_RS20885
• Protein ID: WP_000554757.1
• Coordinates: 3987680..3987973 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG48_RS20850	3982279..3983523	-	1245	WP_073464911.1	esterase FrsA	-
-	3982939..3983019	-	81	NuclAT_10	-	-
-	3982939..3983019	-	81	NuclAT_10	-	-
-	3982939..3983019	-	81	NuclAT_10	-	-
-	3982939..3983019	-	81	NuclAT_10	-	-
RG48_RS20855	3983615..3984073	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG48_RS20860	3984334..3985791	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
RG48_RS20865	3985848..3986369	-	522	Protein_3784	peptide chain release factor H	-
RG48_RS20870	3986368..3986571	-	204	Protein_3785	RNA ligase RtcB family protein	-
RG48_RS20875	3986817..3987269	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG48_RS20880	3987279..3987677	-	399	WP_073464912.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG48_RS20885	3987680..3987973	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG48_RS20890	3988025..3989080	-	1056	WP_001226164.1	DNA polymerase IV	-
RG48_RS20895	3989151..3989936	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
RG48_RS20900	3989908..3991620	+	1713	Protein_3791	flagellar biosynthesis protein FlhA	-
RG48_RS20915	3991844..3992341	-	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagW/ecpD / yagV/ecpE / gmhA/lpcA	3889885..4001841	111956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.81 Da
• Isoelectric Point: 7.4215

>T51054 WP_073464912.1 NZ_CP010170:c3987677-3987279 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNDPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51054 NZ_CP010170:c3987677-3987279 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATGATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51054 WP_000554757.1 NZ_CP010170:c3987973-3987680 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51054 NZ_CP010170:c3987973-3987680 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6