Detailed information of TA system TA051025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2201899..2202593
• Replicon: chromosome
• Accession: NZ_CP010169
• Organism: Escherichia coli strain H5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: RG47_RS11275
• Protein ID: WP_073464912.1
• Coordinates: 2202195..2202593 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG47_RS11270
• Protein ID: WP_000554757.1
• Coordinates: 2201899..2202192 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG47_RS11240	2197531..2198028	+	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-
RG47_RS11255	2198252..2199964	-	1713	Protein_2026	flagellar biosynthesis protein FlhA	-
RG47_RS11260	2199936..2200721	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
RG47_RS11265	2200792..2201847	+	1056	WP_001226164.1	DNA polymerase IV	-
RG47_RS11270	2201899..2202192	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG47_RS11275	2202195..2202593	+	399	WP_073464912.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG47_RS11280	2202603..2203055	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG47_RS11285	2203301..2203504	+	204	Protein_2032	RNA ligase RtcB family protein	-
RG47_RS11290	2203503..2204024	+	522	Protein_2033	peptide chain release factor H	-
RG47_RS11295	2204081..2205538	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
RG47_RS11300	2205799..2206257	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG47_RS11305	2206349..2207593	+	1245	WP_073464911.1	esterase FrsA	-
-	2206853..2206933	+	81	NuclAT_10	-	-
-	2206853..2206933	+	81	NuclAT_10	-	-
-	2206853..2206933	+	81	NuclAT_10	-	-
-	2206853..2206933	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2188833..2280070	91237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.81 Da
• Isoelectric Point: 7.4215

>T51025 WP_073464912.1 NZ_CP010169:2202195-2202593 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNDPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51025 NZ_CP010169:2202195-2202593 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATGATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51025 WP_000554757.1 NZ_CP010169:2201899-2202192 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51025 NZ_CP010169:2201899-2202192 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6