Detailed information of TA system TA051016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4484116..4484915
• Replicon: chromosome
• Accession: NZ_CP010167
• Organism: Escherichia coli strain H3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: RG46_RS23200
• Protein ID: WP_000347266.1
• Coordinates: 4484451..4484915 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: RG46_RS23195
• Protein ID: WP_001307405.1
• Coordinates: 4484116..4484451 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG46_RS23180	4479901..4480671	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
RG46_RS23185	4480687..4482021	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
RG46_RS23190	4482396..4483967	+	1572	WP_001273741.1	galactarate dehydratase	-
RG46_RS23195	4484116..4484451	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
RG46_RS23200	4484451..4484915	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
RG46_RS23205	4484970..4485779	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
RG46_RS23215	4486028..4487308	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
RG46_RS23220	4487331..4487804	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
RG46_RS23225	4487815..4488594	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
RG46_RS23230	4488584..4489462	+	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
RG46_RS23235	4489480..4489914	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4473460..4484915	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T51016 WP_000347266.1 NZ_CP010167:4484451-4484915 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T51016 NZ_CP010167:4484451-4484915 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT51016 WP_001307405.1 NZ_CP010167:4484116-4484451 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT51016 NZ_CP010167:4484116-4484451 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7