Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3307977..3308479 Replicon chromosome
Accession NZ_CP010167
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG46_RS17270 Protein ID WP_000767829.1
Coordinates 3307977..3308231 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG46_RS17275 Protein ID WP_001259255.1
Coordinates 3308228..3308479 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG46_RS17245 3302992..3303219 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
RG46_RS17250 3303233..3304291 - 1059 WP_000492315.1 transport protein YeeE -
RG46_RS17255 3304470..3305828 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG46_RS25110 3305818..3305880 - 63 WP_010723108.1 membrane protein YoeI -
RG46_RS17260 3306095..3307024 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG46_RS17265 3307070..3307894 - 825 WP_000754749.1 SDR family oxidoreductase -
RG46_RS17270 3307977..3308231 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG46_RS17275 3308228..3308479 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG46_RS17280 3308762..3308812 + 51 WP_001364200.1 his operon leader peptide -
RG46_RS17290 3308958..3309857 + 900 WP_000131776.1 ATP phosphoribosyltransferase -
RG46_RS17295 3309863..3311167 + 1305 WP_032359574.1 histidinol dehydrogenase -
RG46_RS17300 3311164..3312234 + 1071 WP_001572811.1 histidinol-phosphate transaminase -
RG46_RS17305 3312231..3313298 + 1068 WP_021579314.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51012 WP_000767829.1 NZ_CP010167:c3308231-3307977 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51012 NZ_CP010167:c3308231-3307977 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51012 WP_001259255.1 NZ_CP010167:c3308479-3308228 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51012 NZ_CP010167:c3308479-3308228 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References