Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1439712..1440253 Replicon chromosome
Accession NZ_CP010167
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag RG46_RS07345 Protein ID WP_000615982.1
Coordinates 1439712..1439990 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG46_RS07350 Protein ID WP_000729704.1
Coordinates 1439993..1440253 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG46_RS07325 1437294..1437872 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
RG46_RS07335 1438078..1438845 + 768 WP_000333380.1 class II glutamine amidotransferase -
RG46_RS07340 1438816..1439556 - 741 WP_001225679.1 murein L,D-transpeptidase -
RG46_RS07345 1439712..1439990 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG46_RS07350 1439993..1440253 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG46_RS07355 1440439..1441212 + 774 WP_000543899.1 C40 family peptidase -
RG46_RS07360 1441269..1441625 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RG46_RS07365 1441618..1441896 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RG46_RS07370 1442001..1444094 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
RG46_RS07375 1444078..1445217 - 1140 WP_073520257.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T50997 WP_000615982.1 NZ_CP010167:c1439990-1439712 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T50997 NZ_CP010167:c1439990-1439712 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT50997 WP_000729704.1 NZ_CP010167:c1440253-1439993 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT50997 NZ_CP010167:c1440253-1439993 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References