Detailed information of TA system TA050994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 635553..636155
• Replicon: chromosome
• Accession: NZ_CP010167
• Organism: Escherichia coli strain H3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG46_RS03275
• Protein ID: WP_000897305.1
• Coordinates: 635553..635864 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG46_RS03280
• Protein ID: WP_000356397.1
• Coordinates: 635865..636155 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG46_RS03250	631467..632066	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG46_RS03255	632060..632932	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG46_RS03260	632929..633366	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG46_RS03265	633411..634352	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG46_RS03270	634416..635324	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG46_RS03275	635553..635864	+	312	WP_000897305.1	hypothetical protein	Toxin
RG46_RS03280	635865..636155	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG46_RS03290	636741..636959	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG46_RS03295	637178..637420	+	243	WP_001087409.1	hypothetical protein	-
RG46_RS03305	637750..638679	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG46_RS03310	638676..639311	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG46_RS03315	639308..640210	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50994 WP_000897305.1 NZ_CP010167:635553-635864 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50994 NZ_CP010167:635553-635864 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50994 WP_000356397.1 NZ_CP010167:635865-636155 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50994 NZ_CP010167:635865-636155 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7