Detailed information of TA system TA050987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3841364..3842058
• Replicon: chromosome
• Accession: NZ_CP010163
• Organism: Escherichia coli strain H2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG45_RS21130
• Protein ID: WP_001263493.1
• Coordinates: 3841364..3841762 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG45_RS21135
• Protein ID: WP_000554757.1
• Coordinates: 3841765..3842058 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG45_RS21100	3836364..3837608	-	1245	WP_001381542.1	esterase FrsA	-
-	3837024..3837104	-	81	NuclAT_11	-	-
-	3837024..3837104	-	81	NuclAT_11	-	-
-	3837024..3837104	-	81	NuclAT_11	-	-
-	3837024..3837104	-	81	NuclAT_11	-	-
RG45_RS21105	3837700..3838158	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG45_RS21110	3838419..3839876	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG45_RS21115	3839933..3840454	-	522	Protein_3653	peptide chain release factor H	-
RG45_RS21120	3840453..3840656	-	204	Protein_3654	RNA ligase RtcB family protein	-
RG45_RS21125	3840902..3841354	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG45_RS21130	3841364..3841762	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG45_RS21135	3841765..3842058	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG45_RS21140	3842110..3843165	-	1056	WP_001226164.1	DNA polymerase IV	-
RG45_RS21145	3843236..3844021	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
RG45_RS21150	3843993..3845705	+	1713	Protein_3660	flagellar biosynthesis protein FlhA	-
RG45_RS26850	3845921..3846418	-	498	Protein_3661	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T50987 WP_001263493.1 NZ_CP010163:c3841762-3841364 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T50987 NZ_CP010163:c3841762-3841364 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT50987 WP_000554757.1 NZ_CP010163:c3842058-3841765 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT50987 NZ_CP010163:c3842058-3841765 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6