Detailed information of TA system TA050965

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4547049..4547420
• Replicon: chromosome
• Accession: NZ_CP010160
• Organism: Escherichia coli strain H1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A6D0I920
• Locus tag: RG44_RS24465
• Protein ID: WP_050491434.1
• Coordinates: 4547226..4547420 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4547049..4547227 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG44_RS24440	4543004..4544377	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
RG44_RS24445	4544506..4545441	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG44_RS24450	4545493..4546728	-	1236	WP_073545014.1	site-specific integrase	-
RG44_RS24455	4546730..4546945	-	216	WP_000079604.1	excisionase XisR	-
RG44_RS24460	4547024..4547233	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	4547049..4547227	+	179	NuclAT_0	-	Antitoxin
-	4547049..4547227	+	179	NuclAT_0	-	Antitoxin
-	4547049..4547227	+	179	NuclAT_0	-	Antitoxin
-	4547049..4547227	+	179	NuclAT_0	-	Antitoxin
RG44_RS24465	4547226..4547420	-	195	WP_050491434.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG44_RS24470	4547477..4548286	-	810	WP_000166319.1	recombination protein RecT	-
RG44_RS24475	4548279..4550879	-	2601	WP_073545015.1	exodeoxyribonuclease VIII	-
RG44_RS24480	4550981..4551256	-	276	WP_000632297.1	protein RacC	-
RG44_RS24485	4551331..4551501	-	171	WP_001352098.1	conserved protein, Rac prophage	-
RG44_RS24490	4551501..4551722	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7032.91 Da
• Isoelectric Point: 8.9538

>T50965 WP_050491434.1 NZ_CP010160:c4547420-4547226 [Escherichia coli]
MRYDNIKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T50965 NZ_CP010160:c4547420-4547226 [Escherichia coli]
ATGAGATATGACAATATTAAACCATGTCCATTTTGTGGTTGCCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT50965 NZ_CP010160:4547049-4547227 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A6D0I920

Antitoxin