Detailed information of TA system TA050942

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31851..32277
• Replicon: plasmid A
• Accession: NZ_CP010158
• Organism: Escherichia coli strain D10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG43_RS27200
• Protein ID: WP_001312861.1
• Coordinates: 31851..32009 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32053..32277 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG43_RS27160	26875..27270	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
RG43_RS27165	27369..28058	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
RG43_RS27170	28245..28628	-	384	WP_001151524.1	relaxosome protein TraM	-
RG43_RS27175	28949..29551	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
RG43_RS27180	29848..30669	-	822	WP_001234469.1	DUF945 domain-containing protein	-
RG43_RS27185	30791..30988	-	198	WP_052318758.1	hypothetical protein	-
RG43_RS28590	31102..31188	-	87	WP_032147848.1	hypothetical protein	-
RG43_RS27200	31851..32009	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	32053..32277	-	225	NuclAT_0	-	Antitoxin
-	32053..32277	-	225	NuclAT_0	-	Antitoxin
-	32053..32277	-	225	NuclAT_0	-	Antitoxin
-	32053..32277	-	225	NuclAT_0	-	Antitoxin
RG43_RS29540	32089..32277	+	189	WP_001299721.1	hypothetical protein	-
RG43_RS27210	32289..33008	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
RG43_RS27215	33005..33439	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
RG43_RS27220	33494..35452	-	1959	WP_073507090.1	ParB/RepB/Spo0J family partition protein	-
RG43_RS27225	35511..35744	-	234	WP_000006004.1	DUF905 domain-containing protein	-
RG43_RS27230	35800..36327	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroC / iroD / iroE / iroN / vat	1..128612	128612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50942 WP_001312861.1 NZ_CP010158:c32009-31851 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50942 NZ_CP010158:c32009-31851 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT50942 NZ_CP010158:c32277-32053 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin