Detailed information of TA system TA050936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4648569..4649171
• Replicon: chromosome
• Accession: NZ_CP010157
• Organism: Escherichia coli strain D10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG43_RS24570
• Protein ID: WP_000897305.1
• Coordinates: 4648860..4649171 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG43_RS24565
• Protein ID: WP_000356397.1
• Coordinates: 4648569..4648859 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG43_RS24545	4645071..4645973	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
RG43_RS24550	4645970..4646605	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG43_RS24555	4646602..4647531	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
RG43_RS24560	4647746..4647964	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG43_RS24565	4648569..4648859	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG43_RS24570	4648860..4649171	-	312	WP_000897305.1	hypothetical protein	Toxin
RG43_RS24575	4649400..4650308	+	909	WP_001318161.1	alpha/beta hydrolase	-
RG43_RS24580	4650372..4651313	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG43_RS24585	4651358..4651795	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG43_RS24590	4651792..4652664	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG43_RS24595	4652658..4653257	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50936 WP_000897305.1 NZ_CP010157:c4649171-4648860 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50936 NZ_CP010157:c4649171-4648860 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50936 WP_000356397.1 NZ_CP010157:c4648859-4648569 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50936 NZ_CP010157:c4648859-4648569 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7