Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2702083..2702585 Replicon chromosome
Accession NZ_CP010152
Organism Escherichia coli strain D9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG42_RS14400 Protein ID WP_000767829.1
Coordinates 2702083..2702337 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG42_RS14405 Protein ID WP_001259255.1
Coordinates 2702334..2702585 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG42_RS14375 2697098..2697325 - 228 WP_000234896.1 sulfurtransferase TusA -
RG42_RS14380 2697339..2698397 - 1059 WP_000492339.1 transport protein YeeE -
RG42_RS14385 2698576..2699934 - 1359 WP_073503377.1 putrescine/proton symporter PlaP -
RG42_RS26435 2699924..2699986 - 63 WP_010723108.1 membrane protein YoeI -
RG42_RS14390 2700201..2701130 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG42_RS14395 2701176..2702000 - 825 WP_000754758.1 SDR family oxidoreductase -
RG42_RS14400 2702083..2702337 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG42_RS14405 2702334..2702585 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG42_RS14410 2702868..2702918 + 51 WP_001364200.1 his operon leader peptide -
RG42_RS14420 2703064..2703963 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG42_RS14425 2703969..2705273 + 1305 WP_000009594.1 histidinol dehydrogenase -
RG42_RS14430 2705270..2706340 + 1071 WP_073503378.1 histidinol-phosphate transaminase -
RG42_RS14435 2706340..2707407 + 1068 WP_073503379.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50900 WP_000767829.1 NZ_CP010152:c2702337-2702083 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50900 NZ_CP010152:c2702337-2702083 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50900 WP_001259255.1 NZ_CP010152:c2702585-2702334 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50900 NZ_CP010152:c2702585-2702334 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References