Detailed information of TA system TA050886

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2008626..2008997
• Replicon: chromosome
• Accession: NZ_CP010152
• Organism: Escherichia coli strain D9

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG42_RS10605
• Protein ID: WP_001317028.1
• Coordinates: 2008803..2008997 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2008626..2008804 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG42_RS10580	2004581..2005954	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
RG42_RS10585	2006083..2007018	-	936	WP_073503287.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG42_RS10590	2007070..2008305	-	1236	WP_000040852.1	site-specific integrase	-
RG42_RS10595	2008307..2008522	-	216	WP_000079604.1	excisionase XisR	-
RG42_RS10600	2008601..2008810	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2008626..2008804	+	179	NuclAT_0	-	Antitoxin
-	2008626..2008804	+	179	NuclAT_0	-	Antitoxin
-	2008626..2008804	+	179	NuclAT_0	-	Antitoxin
-	2008626..2008804	+	179	NuclAT_0	-	Antitoxin
RG42_RS10605	2008803..2008997	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG42_RS10610	2009054..2009863	-	810	WP_000166319.1	recombination protein RecT	-
RG42_RS10615	2009856..2012456	-	2601	WP_001532611.1	exodeoxyribonuclease VIII	-
RG42_RS10620	2012558..2012833	-	276	WP_000632297.1	protein RacC	-
RG42_RS10625	2012908..2013078	-	171	WP_001352098.1	conserved protein, Rac prophage	-
RG42_RS10630	2013078..2013299	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2007070..2042026	34956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T50886 WP_001317028.1 NZ_CP010152:c2008997-2008803 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T50886 NZ_CP010152:c2008997-2008803 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT50886 NZ_CP010152:2008626-2008804 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin