Detailed information of TA system TA050853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 303040..303767
• Replicon: chromosome
• Accession: NZ_CP010151
• Organism: Escherichia coli strain D8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: RG41_RS01480
• Protein ID: WP_000547563.1
• Coordinates: 303040..303351 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG41_RS01485
• Protein ID: WP_000126304.1
• Coordinates: 303348..303767 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG41_RS01450	298184..299893	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
RG41_RS01455	299903..300445	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
RG41_RS01460	300445..301212	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
RG41_RS01465	301209..301619	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
RG41_RS01470	301612..302079	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
RG41_RS01475	302122..302877	+	756	WP_001314074.1	hypothetical protein	-
RG41_RS01480	303040..303351	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
RG41_RS01485	303348..303767	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
RG41_RS01490	303859..304287	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
RG41_RS01500	304672..305199	+	528	WP_001078777.1	electron transport protein HydN	-
RG41_RS01505	305352..307604	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	274193..313112	38919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T50853 WP_000547563.1 NZ_CP010151:303040-303351 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T50853 NZ_CP010151:303040-303351 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT50853 WP_000126304.1 NZ_CP010151:303348-303767 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT50853 NZ_CP010151:303348-303767 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22