Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4219802..4220304 Replicon chromosome
Accession NZ_CP010150
Organism Escherichia coli strain D7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG40_RS21775 Protein ID WP_000767829.1
Coordinates 4220050..4220304 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG40_RS21770 Protein ID WP_001259255.1
Coordinates 4219802..4220053 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG40_RS21740 4214979..4216046 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG40_RS21745 4216046..4217116 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG40_RS21750 4217113..4218417 - 1305 WP_001317939.1 histidinol dehydrogenase -
RG40_RS21755 4218423..4219322 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG40_RS21765 4219468..4219518 - 51 WP_001364200.1 his operon leader peptide -
RG40_RS21770 4219802..4220053 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG40_RS21775 4220050..4220304 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG40_RS21780 4220387..4221211 + 825 WP_000754758.1 SDR family oxidoreductase -
RG40_RS21785 4221257..4222186 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG40_RS25740 4222401..4222463 + 63 WP_010723108.1 membrane protein YoeI -
RG40_RS21790 4222453..4223811 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG40_RS21795 4223990..4225048 + 1059 WP_000492346.1 transport protein YeeE -
RG40_RS21800 4225062..4225289 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50842 WP_000767829.1 NZ_CP010150:4220050-4220304 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50842 NZ_CP010150:4220050-4220304 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50842 WP_001259255.1 NZ_CP010150:4219802-4220053 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50842 NZ_CP010150:4219802-4220053 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References