Detailed information of TA system TA050837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2161427..2162029
• Replicon: chromosome
• Accession: NZ_CP010150
• Organism: Escherichia coli strain D7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG40_RS11285
• Protein ID: WP_000897305.1
• Coordinates: 2161718..2162029 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG40_RS11280
• Protein ID: WP_000356397.1
• Coordinates: 2161427..2161717 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG40_RS11245	2157372..2158274	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
RG40_RS11250	2158271..2158906	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG40_RS11255	2158903..2159832	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG40_RS11265	2160162..2160404	-	243	WP_001086388.1	hypothetical protein	-
RG40_RS11270	2160623..2160841	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG40_RS11280	2161427..2161717	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG40_RS11285	2161718..2162029	-	312	WP_000897305.1	hypothetical protein	Toxin
RG40_RS11290	2162258..2163166	+	909	WP_001162704.1	alpha/beta hydrolase	-
RG40_RS11295	2163230..2164171	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG40_RS11300	2164216..2164653	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG40_RS11305	2164650..2165522	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG40_RS11310	2165516..2166115	-	600	WP_001295269.1	glucose-1-phosphatase	-
RG40_RS11315	2166214..2166999	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50837 WP_000897305.1 NZ_CP010150:c2162029-2161718 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50837 NZ_CP010150:c2162029-2161718 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50837 WP_000356397.1 NZ_CP010150:c2161717-2161427 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50837 NZ_CP010150:c2161717-2161427 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7