Detailed information of TA system TA050821

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22055..22309
• Replicon: plasmid A
• Accession: NZ_CP010149
• Organism: Escherichia coli strain D6

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: RG39_RS24360
• Protein ID: WP_001351576.1
• Coordinates: 22055..22261 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22248..22309 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG39_RS24315	17607..18008	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
RG39_RS26710	17941..18198	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
RG39_RS24325	18291..18944	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
RG39_RS24335	19883..20740	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
RG39_RS24340	20733..21215	-	483	WP_001273588.1	hypothetical protein	-
RG39_RS24345	21208..21282	-	75	WP_001442103.1	RepA leader peptide Tap	-
RG39_RS24355	21514..21771	-	258	WP_000083845.1	replication regulatory protein RepA	-
RG39_RS24360	22055..22261	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	22248..22309	+	62	NuclAT_1	-	Antitoxin
-	22248..22309	+	62	NuclAT_1	-	Antitoxin
-	22248..22309	+	62	NuclAT_1	-	Antitoxin
-	22248..22309	+	62	NuclAT_1	-	Antitoxin
RG39_RS26860	22565..22639	-	75	Protein_29	endonuclease	-
RG39_RS24370	22885..23097	-	213	WP_001299730.1	ANR family transcriptional regulator	-
RG39_RS24375	23233..23793	-	561	WP_000139315.1	fertility inhibition protein FinO	-
RG39_RS24380	23896..24756	-	861	WP_000704514.1	alpha/beta hydrolase	-
RG39_RS24385	24815..25561	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-14 / blaTEM-1B / sitABCD / aac(3)-IId / aadA5 / qacE / sul1 / mph(A) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..199494	199494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T50821 WP_001351576.1 NZ_CP010149:c22261-22055 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T50821 NZ_CP010149:c22261-22055 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT50821 NZ_CP010149:22248-22309 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin