Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3998609..3999111 Replicon chromosome
Accession NZ_CP010148
Organism Escherichia coli strain D6

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG39_RS20445 Protein ID WP_000767829.1
Coordinates 3998857..3999111 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG39_RS20440 Protein ID WP_001259255.1
Coordinates 3998609..3998860 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG39_RS20410 3993788..3994855 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG39_RS20415 3994855..3995925 - 1071 WP_032151756.1 histidinol-phosphate transaminase -
RG39_RS20420 3995922..3997226 - 1305 WP_024261505.1 histidinol dehydrogenase -
RG39_RS20425 3997232..3998131 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG39_RS20435 3998277..3998327 - 51 WP_001364200.1 his operon leader peptide -
RG39_RS20440 3998609..3998860 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG39_RS20445 3998857..3999111 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG39_RS20450 3999194..4000018 + 825 WP_000754749.1 SDR family oxidoreductase -
RG39_RS20455 4000064..4000993 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG39_RS26420 4001208..4001270 + 63 WP_010723108.1 membrane protein YoeI -
RG39_RS20460 4001260..4002618 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG39_RS20465 4002797..4003855 + 1059 WP_032151755.1 transport protein YeeE -
RG39_RS20470 4003869..4004096 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50811 WP_000767829.1 NZ_CP010148:3998857-3999111 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50811 NZ_CP010148:3998857-3999111 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50811 WP_001259255.1 NZ_CP010148:3998609-3998860 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50811 NZ_CP010148:3998609-3998860 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References