Detailed information of TA system TA050792

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47070..47340
• Replicon: plasmid B
• Accession: NZ_CP010147
• Organism: Escherichia coli strain D5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG38_RS26245
• Protein ID: WP_001312861.1
• Coordinates: 47182..47340 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47070..47133 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG38_RS26200	42462..43433	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
RG38_RS26210	44400..44606	+	207	WP_000275856.1	hypothetical protein	-
RG38_RS26215	44632..45171	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
RG38_RS26220	45239..45472	+	234	WP_000005987.1	DUF905 family protein	-
RG38_RS26225	45500..45697	+	198	Protein_41	hypothetical protein	-
RG38_RS26230	45752..46186	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
RG38_RS26235	46183..46902	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
RG38_RS27870	46914..47102	-	189	WP_001299721.1	hypothetical protein	-
-	46914..47138	+	225	NuclAT_0	-	-
-	46914..47138	+	225	NuclAT_0	-	-
-	46914..47138	+	225	NuclAT_0	-	-
-	46914..47138	+	225	NuclAT_0	-	-
-	47070..47133	-	64	-	-	Antitoxin
RG38_RS26245	47182..47340	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG38_RS26265	48261..48548	+	288	WP_000107535.1	hypothetical protein	-
RG38_RS26270	48666..49487	+	822	WP_001234426.1	DUF945 domain-containing protein	-
RG38_RS26275	49784..50386	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
RG38_RS26285	50707..51090	+	384	WP_001151524.1	relaxosome protein TraM	-
RG38_RS26290	51277..51966	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB	1..88754	88754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50792 WP_001312861.1 NZ_CP010147:47182-47340 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50792 NZ_CP010147:47182-47340 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT50792 NZ_CP010147:c47133-47070 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin