Detailed information of TA system TA050784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3481523..3482125
• Replicon: chromosome
• Accession: NZ_CP010145
• Organism: Escherichia coli strain D5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG38_RS18265
• Protein ID: WP_000897305.1
• Coordinates: 3481523..3481834 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG38_RS18270
• Protein ID: WP_000356397.1
• Coordinates: 3481835..3482125 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG38_RS18235	3476553..3477338	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG38_RS18240	3477437..3478036	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG38_RS18245	3478030..3478902	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG38_RS18250	3478899..3479336	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG38_RS18255	3479381..3480322	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG38_RS18260	3480386..3481294	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG38_RS18265	3481523..3481834	+	312	WP_000897305.1	hypothetical protein	Toxin
RG38_RS18270	3481835..3482125	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG38_RS26995	3482734..3483431	+	698	Protein_3291	IS1 family transposase	-
RG38_RS18300	3483507..3483725	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
RG38_RS18305	3483945..3484187	+	243	WP_001087409.1	hypothetical protein	-
RG38_RS18315	3484517..3485446	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG38_RS18320	3485443..3486078	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG38_RS18325	3486075..3486977	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T50784 WP_000897305.1 NZ_CP010145:3481523-3481834 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T50784 NZ_CP010145:3481523-3481834 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT50784 WP_000356397.1 NZ_CP010145:3481835-3482125 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT50784 NZ_CP010145:3481835-3482125 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7