Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1421432..1421934 Replicon chromosome
Accession NZ_CP010145
Organism Escherichia coli strain D5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG38_RS07620 Protein ID WP_000767829.1
Coordinates 1421432..1421686 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG38_RS07625 Protein ID WP_001259255.1
Coordinates 1421683..1421934 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG38_RS07595 1416447..1416674 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG38_RS07600 1416688..1417746 - 1059 WP_000492330.1 transport protein YeeE -
RG38_RS07605 1417925..1419283 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG38_RS27375 1419273..1419335 - 63 WP_010723108.1 membrane protein YoeI -
RG38_RS07610 1419550..1420479 - 930 WP_073511512.1 LysR family transcriptional regulator -
RG38_RS07615 1420525..1421349 - 825 WP_000754737.1 SDR family oxidoreductase -
RG38_RS07620 1421432..1421686 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG38_RS07625 1421683..1421934 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG38_RS07630 1422218..1422268 + 51 WP_001364200.1 his operon leader peptide -
RG38_RS07640 1422414..1423313 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG38_RS07645 1423319..1424623 + 1305 WP_021571897.1 histidinol dehydrogenase -
RG38_RS07650 1424620..1425690 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
RG38_RS07655 1425690..1426757 + 1068 WP_073511513.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50778 WP_000767829.1 NZ_CP010145:c1421686-1421432 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50778 NZ_CP010145:c1421686-1421432 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50778 WP_001259255.1 NZ_CP010145:c1421934-1421683 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50778 NZ_CP010145:c1421934-1421683 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References