Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2534356..2534858 Replicon chromosome
Accession NZ_CP010143
Organism Escherichia coli strain D4

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG37_RS13550 Protein ID WP_000767829.1
Coordinates 2534356..2534610 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG37_RS13555 Protein ID WP_001259255.1
Coordinates 2534607..2534858 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG37_RS13525 2529371..2529598 - 228 WP_000234896.1 sulfurtransferase TusA -
RG37_RS13530 2529612..2530670 - 1059 WP_000492339.1 transport protein YeeE -
RG37_RS13535 2530849..2532207 - 1359 WP_073503377.1 putrescine/proton symporter PlaP -
RG37_RS26865 2532197..2532259 - 63 WP_010723108.1 membrane protein YoeI -
RG37_RS13540 2532474..2533403 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG37_RS13545 2533449..2534273 - 825 WP_000754758.1 SDR family oxidoreductase -
RG37_RS13550 2534356..2534610 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG37_RS13555 2534607..2534858 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG37_RS13560 2535141..2535191 + 51 WP_001364200.1 his operon leader peptide -
RG37_RS13570 2535337..2536236 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG37_RS13575 2536242..2537546 + 1305 WP_000009594.1 histidinol dehydrogenase -
RG37_RS13580 2537543..2538613 + 1071 WP_073503378.1 histidinol-phosphate transaminase -
RG37_RS13585 2538613..2539680 + 1068 WP_073503379.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50756 WP_000767829.1 NZ_CP010143:c2534610-2534356 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50756 NZ_CP010143:c2534610-2534356 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50756 WP_001259255.1 NZ_CP010143:c2534858-2534607 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50756 NZ_CP010143:c2534858-2534607 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References