Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 656212..656906 Replicon chromosome
Accession NZ_CP010143
Organism Escherichia coli strain D4

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag RG37_RS03375 Protein ID WP_001263488.1
Coordinates 656508..656906 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag RG37_RS03370 Protein ID WP_000554758.1
Coordinates 656212..656505 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG37_RS03345 651844..652341 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
RG37_RS03355 652565..654277 - 1713 Protein_595 flagellar biosynthesis protein FlhA -
RG37_RS03360 654249..655034 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
RG37_RS03365 655105..656160 + 1056 WP_001226164.1 DNA polymerase IV -
RG37_RS03370 656212..656505 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
RG37_RS03375 656508..656906 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
RG37_RS03380 656916..657368 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
RG37_RS03390 657686..657892 + 207 Protein_601 RtcB family protein -
RG37_RS03395 657888..658409 + 522 Protein_602 peptide chain release factor H -
RG37_RS03400 658466..659923 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
RG37_RS03405 660184..660642 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 661238..661318 + 81 NuclAT_12 - -
- 661238..661318 + 81 NuclAT_12 - -
- 661238..661318 + 81 NuclAT_12 - -
- 661238..661318 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T50740 WP_001263488.1 NZ_CP010143:656508-656906 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T50740 NZ_CP010143:656508-656906 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT50740 WP_000554758.1 NZ_CP010143:656212-656505 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT50740 NZ_CP010143:656212-656505 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References