Detailed information of TA system TA050732

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35536..35800
• Replicon: plasmid B
• Accession: NZ_CP010142
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: RG36_RS27650
• Protein ID: WP_001387489.1
• Coordinates: 35648..35800 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	35536..35596 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS27635	31638..32708	-	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-
RG36_RS27640	32727..33935	-	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
-	34115..34172	-	58	NuclAT_1	-	-
-	34115..34172	-	58	NuclAT_1	-	-
-	34115..34172	-	58	NuclAT_1	-	-
-	34115..34172	-	58	NuclAT_1	-	-
RG36_RS27645	34242..35333	-	1092	WP_000426061.1	hypothetical protein	-
-	35536..35596	-	61	NuclAT_0	-	Antitoxin
-	35536..35596	-	61	NuclAT_0	-	Antitoxin
-	35536..35596	-	61	NuclAT_0	-	Antitoxin
-	35536..35596	-	61	NuclAT_0	-	Antitoxin
RG36_RS27650	35648..35800	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
RG36_RS27655	35872..36123	-	252	WP_001291964.1	hypothetical protein	-
RG36_RS27660	36423..36719	+	297	WP_011264046.1	hypothetical protein	-
RG36_RS29550	36784..36960	-	177	WP_001054900.1	hypothetical protein	-
RG36_RS27675	37352..37561	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
RG36_RS27680	37633..38295	-	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
RG36_RS27685	38366..40534	-	2169	WP_015508354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B	-	1..90974	90974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T50732 WP_001387489.1 NZ_CP010142:35648-35800 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T50732 NZ_CP010142:35648-35800 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT50732 NZ_CP010142:c35596-35536 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin