Detailed information of TA system TA050728

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 157048..157302
• Replicon: plasmid A
• Accession: NZ_CP010141
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: RG36_RS27245
• Protein ID: WP_001351576.1
• Coordinates: 157096..157302 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	157048..157109 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS27220	153796..154542	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
RG36_RS27225	154601..155461	+	861	WP_000704514.1	alpha/beta hydrolase	-
RG36_RS27230	155564..156124	+	561	WP_000139315.1	fertility inhibition protein FinO	-
RG36_RS27235	156260..156472	+	213	WP_001299730.1	ANR family transcriptional regulator	-
RG36_RS29525	156718..156792	+	75	Protein_163	endonuclease	-
-	157048..157109	-	62	NuclAT_1	-	Antitoxin
-	157048..157109	-	62	NuclAT_1	-	Antitoxin
-	157048..157109	-	62	NuclAT_1	-	Antitoxin
-	157048..157109	-	62	NuclAT_1	-	Antitoxin
RG36_RS27245	157096..157302	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG36_RS27250	157586..157843	+	258	WP_000083845.1	replication regulatory protein RepA	-
RG36_RS27260	158075..158149	+	75	WP_001442103.1	RepA leader peptide Tap	-
RG36_RS27265	158142..158624	+	483	WP_001273588.1	hypothetical protein	-
RG36_RS27270	158617..159180	+	564	Protein_168	incFII family plasmid replication initiator RepA	-
RG36_RS27275	159372..160913	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
RG36_RS27280	160928..161674	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
RG36_RS27285	161761..162060	+	300	Protein_171	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / blaTEM-1B / tet(B) / catA1 / aadA5 / qacE / sul1 / blaCTX-M-55 / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..174041	174041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T50728 WP_001351576.1 NZ_CP010141:157096-157302 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T50728 NZ_CP010141:157096-157302 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT50728 NZ_CP010141:c157109-157048 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin