Detailed information of TA system TA050724

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 118704..118973
• Replicon: plasmid A
• Accession: NZ_CP010141
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG36_RS27005
• Protein ID: WP_001312861.1
• Coordinates: 118815..118973 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	118704..118769 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS26960	113923..114894	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
RG36_RS29520	116033..116239	+	207	WP_000275856.1	hypothetical protein	-
RG36_RS26975	116265..116804	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
RG36_RS26980	116872..117105	+	234	WP_000005987.1	DUF905 family protein	-
RG36_RS26985	117133..117330	+	198	Protein_116	hypothetical protein	-
RG36_RS26990	117385..117819	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
RG36_RS26995	117816..118535	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
RG36_RS29385	118547..118735	-	189	WP_001299721.1	hypothetical protein	-
-	118547..118771	+	225	NuclAT_0	-	-
-	118547..118771	+	225	NuclAT_0	-	-
-	118547..118771	+	225	NuclAT_0	-	-
-	118547..118771	+	225	NuclAT_0	-	-
-	118704..118769	+	66	-	-	Antitoxin
RG36_RS27005	118815..118973	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG36_RS27025	119894..120181	+	288	WP_000107535.1	hypothetical protein	-
RG36_RS27030	120299..121120	+	822	WP_001234426.1	DUF945 domain-containing protein	-
RG36_RS27035	121417..122019	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
RG36_RS27045	122340..122723	+	384	WP_001151524.1	relaxosome protein TraM	-
RG36_RS27050	122910..123599	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / blaTEM-1B / tet(B) / catA1 / aadA5 / qacE / sul1 / blaCTX-M-55 / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..174041	174041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50724 WP_001312861.1 NZ_CP010141:118815-118973 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50724 NZ_CP010141:118815-118973 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT50724 NZ_CP010141:118704-118769 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin