50719

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3913680..3914373 Replicon chromosome
Accession NZ_CP010140
Organism Escherichia coli strain D3

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag RG36_RS20640 Protein ID WP_000415584.1
Coordinates 3914077..3914373 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag RG36_RS20635 Protein ID WP_000650107.1
Coordinates 3913680..3914075 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG36_RS20625 3909544..3911802 - 2259 WP_001281841.1 DNA topoisomerase IV subunit A -
RG36_RS20630 3911940..3913547 - 1608 WP_001324265.1 ABC transporter substrate-binding protein -
RG36_RS20635 3913680..3914075 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
RG36_RS20640 3914077..3914373 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
RG36_RS20645 3914578..3915060 - 483 WP_000183494.1 transcriptional regulator -
RG36_RS20650 3915113..3915505 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
RG36_RS20655 3915657..3916316 + 660 WP_001221491.1 two-component system response regulator QseB -
RG36_RS20660 3916313..3917662 + 1350 WP_000673406.1 two-component system sensor histidine kinase QseC -
RG36_RS20665 3917772..3918353 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
RG36_RS20670 3918384..3918698 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T50719 WP_000415584.1 NZ_CP010140:c3914373-3914077 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T50719 NZ_CP010140:c3914373-3914077 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT50719 WP_000650107.1 NZ_CP010140:c3914075-3913680 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT50719 NZ_CP010140:c3914075-3913680 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LU65

References