Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3543146..3543873 Replicon chromosome
Accession NZ_CP010140
Organism Escherichia coli strain D3

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag RG36_RS18730 Protein ID WP_000547564.1
Coordinates 3543562..3543873 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag RG36_RS18725 Protein ID WP_000126294.1
Coordinates 3543146..3543565 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG36_RS18705 3538196..3538723 - 528 WP_001078777.1 electron transport protein HydN -
RG36_RS18710 3538872..3539882 - 1011 WP_001363554.1 DNA-binding transcriptional regulator AscG -
RG36_RS18715 3540142..3541599 + 1458 WP_001107856.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
RG36_RS18720 3541608..3543032 + 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
RG36_RS18725 3543146..3543565 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
RG36_RS18730 3543562..3543873 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
RG36_RS18735 3544047..3544808 - 762 WP_001026446.1 hypothetical protein -
RG36_RS18740 3544833..3545303 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
RG36_RS18745 3545296..3545706 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
RG36_RS18750 3545703..3546470 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
RG36_RS18755 3546470..3547012 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
RG36_RS18760 3547022..3548731 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T50716 WP_000547564.1 NZ_CP010140:c3543873-3543562 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T50716 NZ_CP010140:c3543873-3543562 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT50716 WP_000126294.1 NZ_CP010140:c3543565-3543146 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT50716 NZ_CP010140:c3543565-3543146 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5


Antitoxin

Source ID Structure

References