Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2749357..2749859 Replicon chromosome
Accession NZ_CP010140
Organism Escherichia coli strain D3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG36_RS14655 Protein ID WP_000767829.1
Coordinates 2749357..2749611 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG36_RS14660 Protein ID WP_001259255.1
Coordinates 2749608..2749859 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG36_RS14630 2744373..2744600 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG36_RS14635 2744614..2745672 - 1059 WP_000492315.1 transport protein YeeE -
RG36_RS14640 2745851..2747209 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG36_RS28865 2747199..2747261 - 63 WP_010723108.1 membrane protein YoeI -
RG36_RS14645 2747475..2748404 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG36_RS14650 2748450..2749274 - 825 WP_000754758.1 SDR family oxidoreductase -
RG36_RS14655 2749357..2749611 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG36_RS14660 2749608..2749859 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG36_RS14665 2750142..2750192 + 51 WP_001364200.1 his operon leader peptide -
RG36_RS14675 2750338..2751237 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG36_RS14680 2751243..2752547 + 1305 WP_001774536.1 histidinol dehydrogenase -
RG36_RS14685 2752544..2753614 + 1071 WP_001362957.1 histidinol-phosphate transaminase -
RG36_RS14690 2753614..2754681 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50714 WP_000767829.1 NZ_CP010140:c2749611-2749357 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50714 NZ_CP010140:c2749611-2749357 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50714 WP_001259255.1 NZ_CP010140:c2749859-2749608 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50714 NZ_CP010140:c2749859-2749608 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References