50714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2749357..2749859 Replicon chromosome
Accession NZ_CP010140
Organism Escherichia coli strain D3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG36_RS14655 Protein ID WP_000767829.1
Coordinates 2749357..2749611 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG36_RS14660 Protein ID WP_001259255.1
Coordinates 2749608..2749859 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG36_RS14630 2744373..2744600 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG36_RS14635 2744614..2745672 - 1059 WP_000492315.1 transport protein YeeE -
RG36_RS14640 2745851..2747209 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG36_RS28865 2747199..2747261 - 63 WP_010723108.1 membrane protein YoeI -
RG36_RS14645 2747475..2748404 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG36_RS14650 2748450..2749274 - 825 WP_000754758.1 SDR family oxidoreductase -
RG36_RS14655 2749357..2749611 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG36_RS14660 2749608..2749859 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG36_RS14665 2750142..2750192 + 51 WP_001364200.1 his operon leader peptide -
RG36_RS14675 2750338..2751237 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG36_RS14680 2751243..2752547 + 1305 WP_001774536.1 histidinol dehydrogenase -
RG36_RS14685 2752544..2753614 + 1071 WP_001362957.1 histidinol-phosphate transaminase -
RG36_RS14690 2753614..2754681 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T50714 WP_000767829.1 NZ_CP010140:c2749611-2749357 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T50714 NZ_CP010140:c2749611-2749357 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT50714 WP_001259255.1 NZ_CP010140:c2749859-2749608 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT50714 NZ_CP010140:c2749859-2749608 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References