Detailed information of TA system TA050701

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1931747..1932118
• Replicon: chromosome
• Accession: NZ_CP010140
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A1Y2ZGM7
• Locus tag: RG36_RS10235
• Protein ID: WP_042038331.1
• Coordinates: 1931924..1932118 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1931747..1931925 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS10210	1927702..1929075	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
RG36_RS10215	1929204..1930139	-	936	WP_001157405.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG36_RS10220	1930191..1931426	-	1236	WP_000040851.1	site-specific integrase	-
RG36_RS10225	1931428..1931643	-	216	WP_000079604.1	excisionase XisR	-
RG36_RS10230	1931743..1931931	-	189	WP_001302840.1	DUF1187 family protein	-
-	1931747..1931925	+	179	NuclAT_0	-	Antitoxin
-	1931747..1931925	+	179	NuclAT_0	-	Antitoxin
-	1931747..1931925	+	179	NuclAT_0	-	Antitoxin
-	1931747..1931925	+	179	NuclAT_0	-	Antitoxin
RG36_RS10235	1931924..1932118	-	195	WP_042038331.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG36_RS10240	1932175..1932984	-	810	WP_000166319.1	recombination protein RecT	-
RG36_RS10245	1932977..1935577	-	2601	WP_069354050.1	exodeoxyribonuclease VIII	-
RG36_RS10250	1935679..1935953	-	275	Protein_1824	hypothetical protein	-
RG36_RS10255	1936028..1936198	-	171	WP_001485242.1	hypothetical protein	-
RG36_RS10260	1936198..1936419	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1930191..1978248	48057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.94 Da
• Isoelectric Point: 8.9538

>T50701 WP_042038331.1 NZ_CP010140:c1932118-1931924 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T50701 NZ_CP010140:c1932118-1931924 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGTCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAACGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT50701 NZ_CP010140:1931747-1931925 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1Y2ZGM7

Antitoxin